Delivery of chemical cargo to endogenous proteins on live cells
نویسنده
چکیده
During the past two decades, our collective mastery of gene manipulation, delivery, and fluorescent fusion protein expression has been astounding and has enabled never before dreamed of new lines of research. These advances, coupled with progress in the collection and analysis of microscopy data, have allowed the scientific community to detect proteins in live cells with resolution that was unthinkable not long ago. The confluence of protein engineering and advanced microscopy has enabled us to observe cellular processes at the single molecule level and to begin unraveling the mysteries of cellular biochemistry and dynamics. These advances, however, require delivery of a contrast agent to the subject organism which can sometimes lead to untoward effects and potentially confounded results. Here, I present an opinion on the need to improve and deploy new strategies that allow for the silent tagging of endogenous proteins with contrast agents.
منابع مشابه
Bone Tissue Engineering: a Mini-Review
Despite advances in bone tissue engineering, auto grafts from intra-oral or extra-oral donor sites are still the gold standard for treatment of large craniomaxillofacial defects. Biomaterial development, application of growth factor, and stem cells, open new gateway to bone regeneration studies, but real translation from bench to bedside have not yet happened. In this review article, a number o...
متن کاملExosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein–protein interaction module
Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named 'exosomes for protein loadin...
متن کاملGenomic targeting of epigenetic probes using a chemically tailored Cas9 system.
Recent advances in the field of programmable DNA-binding proteins have led to the development of facile methods for genomic localization of genetically encodable entities. Despite the extensive utility of these tools, locus-specific delivery of synthetic molecules remains limited by a lack of adequate technologies. Here we combine the flexibility of chemical synthesis with the specificity of a ...
متن کاملDiscovery and characterization of a peptide that enhances endosomal escape of delivered proteins in vitro and in vivo.
The inefficient delivery of proteins into mammalian cells remains a major barrier to realizing the therapeutic potential of many proteins. We and others have previously shown that superpositively charged proteins are efficiently endocytosed and can bring associated proteins and nucleic acids into cells. The vast majority of cargo delivered in this manner, however, remains in endosomes and does ...
متن کاملLive Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals
Neurons are highly polarized cells that require continuous turnover of membrane proteins at axon terminals to develop, function, and survive. Yet, it is still unclear whether membrane protein degradation requires transport back to the cell body or whether degradation also occurs locally at the axon terminal, where live observation of sorting and degradation has remained a challenge. Here, we re...
متن کامل